sema3a elisa kit  (Cusabio)

Journal: International Journal of General Medicine

Article Title: Plasma Semaphorin 3A as a Potential Biomarker for Cognitive Impairment in Cerebral Small Vessel Disease

doi: 10.2147/IJGM.S554075

Figure Lengend Snippet: Violin plot comparing plasma Sema3A levels across diagnostic categories. ( A ) Patients vs HCs. ( B ) Acute phase vs subacute phase in RSSI patients. ( C ) Subacute phase vs HCs. ( D ) CI vs NCI subgroup in patients. ( E ) High-load vs low-load CSVD imaging score subgroups. Abbreviations: HC: healthy control; CI: cognitive impairment; NCI: non-cognitive impairment; CSVD: cerebral small vessel disease. *** P < 0.001.ns: not significant.

Article Snippet: Plasma Sema3A concentrations were measured using a double antibody sandwich enzyme-linked immunosorbent assay (ELISA, Human SEMA3A ELISA Kit, Elabscience, Wuhan, China).

Techniques: Clinical Proteomics, Diagnostic Assay, Imaging, Control

Journal: International Journal of General Medicine

Article Title: Plasma Semaphorin 3A as a Potential Biomarker for Cognitive Impairment in Cerebral Small Vessel Disease

doi: 10.2147/IJGM.S554075

Figure Lengend Snippet: Correlation analysis of plasma Sema3A levels in the acute phase. ( A ) Correlation between plasma Sema3A levels and MoCA score in RSSI patients. ( B ) Correlation between plasma Sema3A levels and total burden score in RSSI patients.

Article Snippet: Plasma Sema3A concentrations were measured using a double antibody sandwich enzyme-linked immunosorbent assay (ELISA, Human SEMA3A ELISA Kit, Elabscience, Wuhan, China).

Techniques: Clinical Proteomics

Journal: International Journal of General Medicine

Article Title: Plasma Semaphorin 3A as a Potential Biomarker for Cognitive Impairment in Cerebral Small Vessel Disease

doi: 10.2147/IJGM.S554075

Figure Lengend Snippet: ROC analysis of plasma Sema3A levels. ( A ) ROC curve comparing RSSI patients and HCs. ( B ) ROC curve comparing CI and NCI subgroups in RSSI patients.

Article Snippet: Plasma Sema3A concentrations were measured using a double antibody sandwich enzyme-linked immunosorbent assay (ELISA, Human SEMA3A ELISA Kit, Elabscience, Wuhan, China).

Techniques: Clinical Proteomics

Journal: International Journal of General Medicine

Article Title: Plasma Semaphorin 3A as a Potential Biomarker for Cognitive Impairment in Cerebral Small Vessel Disease

doi: 10.2147/IJGM.S554075

Figure Lengend Snippet: Mediation analysis of plasma Sema3A, CSVD total burden score, and cognitive function. ( A ) Conceptual diagram of mediation analysis with single mediator (M). Total effect of X on Y (c) = indirect effect through M (a × b) + direct effect (c’). ( B ) Sema3A (X), total burden score (M), MoCA (Y). ( C ) Sema3A (X), LI (M), MoCA (Y). ( D ) Sema3A (X), CMBs (M), MoCA (Y). ( E ) Sema3A (X), EPVs (Potter score) (M), MoCA (Y). ( F ) Sema3A (X), WMH (Fazekas score) (M), MoCA (Y). Covariates: age, sex, education.***P < 0.001.

Article Snippet: Plasma Sema3A concentrations were measured using a double antibody sandwich enzyme-linked immunosorbent assay (ELISA, Human SEMA3A ELISA Kit, Elabscience, Wuhan, China).

Techniques: Clinical Proteomics

Journal: Frontiers in Molecular Neuroscience

Article Title: Basolateral Amygdala Mediates Central Mechanosensory Feedback of Musculoskeletal System

doi: 10.3389/fnmol.2022.834980

Figure Lengend Snippet: Decreased Sema3A secretion with sensory nerve dysfunction in aging and muscle atrophic mice. (A) ELISA of serum Sema3A levels in mice at different ages, i.e., 7, 35, and 68 weeks old. (B) ELISA of serum Sema3A levels after BTxA-induced muscle dysfunction. (C) Chemogenetic activation of BLA CaMKII neurons after muscle paralysis. (D) ELISA of serum Sema3A levels in BTxA model after chemogenetic activation of BLA CaMKII neurons. (E) Representative immunofluorescent staining images of CGRP (green) in femurs of saline- or BTxA-treated mice. Scale bar, 50 μm. (F) Representative immunofluorescent staining images of CGRP (green) in tibia after chemogenetic manipulation of BLA CaMKII neurons. Scale bar, 50 μm. (G) Quantitative analysis of CGRP + -nerve fibers in BTxA-injected mouse models. (H) Quantitative analysis of CGRP + -nerve fibers in hM3Dq vs. mCherry group of BTxA-injected mice. All data represent mean ± SD. Statistical analysis was assessed using student t -tests for panel (D) and one-way ANOVA followed Tukey comparison test for panels (A,B) . * P < 0.05, ** P < 0.01, **** P < 0.0001.

Article Snippet: The serum Sema3A level was detected using Sema3A ELISA kit (CUSABIO, CSB-E15913h) which have been described previously in this study.

Techniques: Enzyme-linked Immunosorbent Assay, Activation Assay, Staining, Saline, Injection, Comparison

Journal: Frontiers in Molecular Neuroscience

Article Title: Basolateral Amygdala Mediates Central Mechanosensory Feedback of Musculoskeletal System

doi: 10.3389/fnmol.2022.834980

Figure Lengend Snippet: Clinical cases for potential application of Sema3A as a prognostic signal of bone loss. (A) Correlation between BMD (T-score) and age (years) in 18 patients. (B) Correlation between BMD (T-score) and Sema3A level in 18 patients. (C) Serum Sema3A level between normal BMD group (T-score > –1) and low BMD group (T-score ≤ –1). (D) Correlation between BMD (T-score) and Sema3A level in 18 patients. (E) Correlation between BMD (T-score) and Sema3A level in male group. (F) Correlation between BMD (T-score) and Sema3A level in female group. For all clinical data, linear regression models were used to explore the correlation between two variables. P < 0.05 was considered significant. (G) Basolateral amygdala (BLA) mediates mechanosensory role of bone in response to mechanical unloading.

Article Snippet: The serum Sema3A level was detected using Sema3A ELISA kit (CUSABIO, CSB-E15913h) which have been described previously in this study.

Techniques: